This technology now referred to as transgenic mouse technology has revolutionized virtually all fields. Mouse transgenic and gene targeting facility transgenic. Softmousedb mouse colony transgenic, knockout, mouse, mice breeding and colony management subscription plan 30 day free trial 1. Moreover, many transgenic mice suitable for cardiovascular. Best practices for efficient mouse colony management. The central entity of the rodentsql module is the mouse id. Patent and licensing issues in transgenic technology. Animal colony management has been an issue of concern for many years 18. Transgenic and targeted mice made by the core 2020. Transgenic mouse colony management current protocols in. Although mice breed readily when left to themselves, it is. After e x t r a c t i o n, the dna is ethanol p r e c i p i t a t e d and r e d i s s o l v e d in 10 mm trishc1, ph 757 production of transgenic mice 45 table iv reagents for transgenic mouse production a concentrated hc1 fisher naoh sigma, st.
Thus, the transgenic mouse colony consists of mice that produce consistent levels of virus that are different for each mouse. Efficient gemm colony management requires adequate recording of breeding and. Softmouse internet colony management database if you are interested in quickly getting up and running on a free trial of softmouse db mouse colony management software then we can save you valuable time and import your data using the softmousedb data import tool service. The prts, in operation since 1995, is administered through the department. The history of mouse genetics is reflected in the early adoption of software for the management and analysis of mouse colonies bishop and hanna 1968. Lasp functions as a comprehensive resource for nci scientists performing animalbased research by providing outstanding veterinarian care, regulatory support, and routine and specialized husbandry services on the ncibethesda and ncifrederick campuses. Softmousedb mouse colony transgenic, knockout, mouse, mice. Jun 06, 2018 it has been these authorss experience, that despite the anticipated value of gene targeted and transgenic animal studies toward this goal, many institutions do not maintain a transgenic mouse colony, in part due to the high costs of genotyping and associated housing of animals during the genotyping delay and extensive record keeping required. The protocol must include pertinent information, including each strain of mouse to be bred, approximate numbers, and the person responsible for the breeding colony. Ideally, a free or relatively inexpensive system used to track and analyze breeding patterns in a transgenic mouse line. C57bl6 mouse model information sheet charles river. Colony managers oft en consult us for advice and rightly so, for our mouse husbandry experts have been using and refi ning mouse colony management techniques for over 75 years. Purpose and background the brown university iacuc has developed this policy to provide guidance to research personnel regarding the housing of mice and management of breeding colonies.
Louis, mo fluorinert 3m, fc77 industrial chemical products, st. Save by purchasing the access to these 3 recordings for your training program. Breeding mice in the laboratory is one method of ensuring an available supply of. Soft mouse db efficiently allows researchers to create, store, manipulate and search records associated with mouse based research, including transgenic mouse generation, mouse breeding and genetics, developmental studies, mouseline colony maintenance, cryopreservation and much more. Lasp also staffs and operates many stateoftheart research cores and. Over four decades of applied animal colony informatics, researchers have progressively developed more flexible computational. Genetic analyses using mice as a model system for human disease are a cornerstone of biomedical research. Every other entity has a relationship with the mouse id which facilitates complete tracking over other parameters like generations, genotype tracking and mouse caging. Some existing tools like microsoft excel sheets and couple other colony management webcloudbased tools are either cumbersome or expensive. Transgenic mouse colony management conner 2005 current. Transgenic mouse colony management conner 2005 current protocols in molecular biology wiley online library. The care of transgenic mouse colonies is an evermoving target. Fielder, ma, established the transgenic mouse facility at the university of california, irvine, in 1996 and managed it for 17 years.
Sox12 enhances fbw7mediated ubiquitination and degradation of gata3 in th2 cells. This unit describes strategies and methods used to establish a colony of transgenic mice. Gene modification the gene modification facility is offering services for gene modification in cell lines and in conjunction with the gene targeting and transgenic mouse facilities for the complete generation of gene targeted mouse lines including the generation of conventional gene knockouts, conditional knockouts, knockin mutations and the generation of tagged proteins. Introduction of foreign dna into the mouse germ line is considered a major technical advancement in the fields of developmental biology and genetics. Auroras guide to mouse colony management at mit aurora burds connor, feb 2007. Transgenic mouse model tests are possible alternatives to the classical 2year cancer bioassay 31. Of the 8,012 transgenic alleles published in the mouse genome database, only 416 5. For transgenic cre alleles, the number is even lower, with a known 19. The primary role of the mtgtf is to support the investigators in using genetically modified mouse models in brown university, affiliated hospitals and academic institutions in rhode island and other states. Onestop solution to generate transgenic mouse models. Overview of todays presentation 2 mouse reproduction factors affecting breeding performance. Only breeding and colony maintenance will be allowed, including genotyping and identification of animals. Hemizygous transgenic male mice with the g93a mutation of the human sod1 gene g1h1 mutation b6sjltgn sod1g93a 1 gur were obtained from jackson laboratories and were bred locally with female b6sjl mice jackson laboratory, bar harbor, me, usa, according to the protocol obtained from the vendor.
Basic mating and genotyping strategies are introduced. Welcome jaxjcms, gmouse, filemaker pro, mouse house and spreadsheet users, switch for free to the 247 supported softmouse forever free or premium cms learn more. The rodentsql application is composed of colony management, experimentprotocol management and report functions. Services include rodent breeding, strategic planning and maintenance, colony maintenance, animal identification, animal ordering, sample collection for genotyping, euthanasia, tracking animal usage numbers, and. Transgenic technology and laboratory animal science ilar. Use this page to register for a forever free mouse colony management software account as well as a premium colony management software database account. Mouse colony management experts 3rd generation internet colony management database and software platform in the cloud.
Policy on mouse housing density and colony management. Advanced protocols for animal transgenesis springerlink. Transgenic rats were initially generated for a neuroscience laboratory at the massachusetts institute of technology mit by riken, japan, with the intention of studying the poliovirus receptorrelated 3 pvrl3 gene in the ca1 region of the hippocampus according to previously published methods. Course manual and courses the jackson laboratory has a great free pdf manual about breeding mice that can be downloaded. You can contract us to perform your entire transgenic project from initial strategy design and dna vector construction to transgenic animal delivery, or you can outsource specific phases of the project to us. However, in the last segment of the manuscript, direct focus on specific aspects of laboratory animal management will be provided. See an example and get a mouse colony management calendar template. Thus, essential to the efficient management and financial survival of a transgenic colony is the ability to rapidly generate and communicate genotyping data, breeding instructions, and breeding productivity between the end users, the animal care staff, and the genotyping staff.
Transgenic mice 28 background controls inbred or mixed background wildtype noncarrier sibling. Maintaining a breeding colony of mice is one method of generating and then ensuring an available supply of experimental subjects with desired characteristics. Jul 27, 2018 it can be easily installed and customized. Transgenic mice custom transgene expression taconic. Gene targetinggene modificationtransgenic mouse ilab. Request pdf transgenic mouse colony management this unit describes strategies and methods used to establish a colony of transgenic mice. Get your taconic biosciences 2020 pricing guide today. Mouse colony and breeding management webinar recording. It was developed by a mouse researcher to satisfy the need for simplicity, usability, and interactivity.
This gramnegative coccobacillus infects a variety of rodents, including mice, rats, kangaroo rats, guinea pigs, hamsters, and gerbils. Adding strains to the colony will require a modification to the protocol. New transgenic and targeted mice that we have created are featured in over 270 publications since 2004. Softmouse db is a webbased mouse colony management system designed for life scientists working with mouse models.
Auroras guide to mouse colony management aurora burds connor, 2006 breeding mice in the laboratory is one method of ensuring an available supply of experimental subjects with desired characteristics. It defines guidelines necessary to establish the colony, and describes screening for germline transmission, marking the mice for easy and consistent identification, and genotyping dna by pcr or southern blotting analysis. Mousej is a free mouse colony management software designed for laboratories. The mtgtf provides services of molecular design and generation of transgenic and knockout mouse. The management of mouse colonies created by gene targeting is described in this unit. Policy on mouse housing density and colony management purpose. View the article pdf and any associated supplements and figures for a period of 48. The rationale for using transgenic mice in regulatory carcinogenicity testing is that transgenic mouse models may be more sensitive predictors of carcinogenic risk to humans. Learn from these three experts on how to manage a breeding colony of transgenic mice. Many research groups still struggle to have central storage and integrated management system of this metadata. Softmouse db subscription plan cdn canadian dollars. The prts, in operation since 1995, is administered through the department of comparative medicine, located in the magnuson. In transgenic mouse methods and protocols, marten hofker and jan van deursen have assembled a multidisciplinary collection of readily reproducible methods for working with mice, and particularly for generating mouse models that will enable us to better understand gene function.
This manual describes breeding strategies and techniques for maintaining colonies. Colony planning its important to determine the number of breeding pairs you need to supply sufficient mice for your experiments. Leading academic labs, biotech and pharmaceutical companies trust softmouse. Most of the mouse experiments tend to generate a largescale data, which is needed to be stored and eventually made publicly available. Abstract this unit describes strategies and methods used to establish a colony of transgenic mice. Microsoft excel spreadsheetbased animal colony management.
Auroras guide to mouse colony management aurora burds connor, 2006. Gordon jw, ruddle fh 1981, integration and stable germ line transmission of genes injected into mouse pronuclei. Free colony and breeding management software for labs on a budget. Largescale discovery of mouse transgenic integration. The forever free edition of softmouse is essentially a simplified version of the fullfeatured premium single user account. From testing rodentsql on a beta user, we found that it helps to reduce the amount of time spent with mouse colony management. Care offers colony management services to all rodent users on a feeforservice basis. Mouse colony management fundamentals webinar recording this presentation covers strategies for selection of animals and breeding schemes, expansion of a colony including calculation examples, practical tips on. George stancel, dean of the graduate school of biomedical science at utmsh. Free online mouse colony management software for labs on a. During this time, the facility conducted numerous inhouse breeding projects for its clients and trained multiple laboratory animal technicians in the basics of colony management.
Take advantage of the convenience of having your husbandry, sourcing, and efficacy evaluation needs met by jax. C57bl6 mouse, c57bl6 mice, c57bl6, c57, c57bl6n, laboratory mice, inbred mice, bl6, transgenic model development, knockout model development created date. Breeding strategies for maintaining colonies of laboratory. Auroras guide to mouse colony management contents handling of. With pronuclear microinjection, a predesigned gene or genes are injected and randomly integrated into the mouse or rat genome. Jax services for mouse colony management 15 dedicated supply, custom breeding, special crosses and stocks. Mouse colony management research animal resources and. This is usually done to propagate mutant or transgenic lines, or because the trait is. In other words, on the softmouse forever free version, some of the features that you are currently trialing now will become disabled for example, the customizable drop down lists and the litter history table in the mating record, however all data that you entered during. Softmousedb academic pricing plans for transgenic mouse. Can anyone recommend a mouse colony management software system. Hepatitis b virus transgenic mouse model of chronic liver.
Ucar guidelines on breeding colonies for maintaining. Can anyone recommend a mouse colony management software. Pasteurella pneumotropica is an opportunistic pathogen that is found in many research and commercial rodent colonies. The expression of a transgene may affect a strains viability. Here, we report a large set of driver and reporter transgenic mouse lines, including 23 new driver lines targeting a variety of cortical and subcortical cell populations and 26 new reporter lines expressing an array of molecular tools. Chen sz, evans ga 1990, a simple screening method for transgenic mice using the polymerase chain reaction. Although mice breed readily when left to themselves, it is helpful to have a working knowledge of. The breeding colony size planning worksheet pdf is a very useful resource.
This manual describes breeding strategies and techniques for maintaining. Below are basic principles for managing small mouse colonies. Cross breeding the mating of animals of different breeds or strains, also called hybrid cross. We are supported by the case western reserve university school of medicine the case comprehensive cancer center, the clinical and translational science collaborative and by grants from the cystic fibrosis foundation. What is softmouse db colony maintenance and breeding management. Breeding strategies for maintaining colonies of laboratory mice. Take the mouse breeding colony management course through rarc training. A suite of transgenic driver and reporter mouse lines with. Th ese techniques are safe, reliable, economical, effi cient, and ensure that the mouse strains produced are genetically welldefi ned. Random transgenic mice taconics custom transgenics program employs pronuclear microinjection technology to generate geneticallymodified founder animals. Several transgenic mouse models have been generated that model mutations found in fals patients see table 1 below for comparisons of key characteristics, including the g93a gurney et al. Analytical genetics and transgenics core p30ar048311. Manage, store, and organize data such as mice, mice cages, mice events, mice related. In need of mouse breeding and colony management expertise to advance your.
Iseehear offers forever free mouse colony management software and premium mouse and rat colony management software. Suehiro ki, suto a, suga k, furuya h, iwata a, iwamoto t, tanaka s, kageyama t, suzuki k, hirose k, lefebvre v, nakajima h. Softmouse db subscription plan welcome to iseehear 2. Internet colony management mouse colony software management. Modern genetic approaches are powerful in providing access to diverse cell types in the brain and facilitating the study of their function. Elimination of pasteurella pneumotropica from a mouse. Bac transgenes, dna purification, and transgenic mouse production.
1104 408 727 334 261 523 428 152 534 764 924 1388 603 1170 171 1449 359 912 1398 536 315 90 828 236 754 654 699 1475 1333 737 474 673 262 586 1066 8